The EU had earlier slammed Google in June with a fine of 2.4-billion euro for illegally favoring its shopping centers in the search results.
Initially Google was given 90 days to comply or face further fines.
Google said it had no further comment.
"The Commission's decision stands on firm ground, both legally and factually, and we expect the Commission to win on appeal", FairSearch lawyer Thomas Vinje said.
At the time of the ruling, Europe's antitrust chief Margrethe Vestager said Google's strategy for attracting customers to its online shopping service was not just about being better than rivals.
Google has made a decision to appeal the record-breaking fine imposed on it by the European Union's highest antitrust authority in July.
Now, Google has filed an appeal with the Court of Justice of the European Union in Luxembourg.
The fine over Google Shopping broke the previous European Union record for a monopoly case against USA chipmaker Intel of 1.06 billion euros in 2009 and made the EU the global leader in regulating Silicon Valley giants.
The EU fined the internet giant for abusing its dominance in Europe to position its own shopping comparison service at the top of Google search results. "And most importantly, it denied European consumers a genuine choice of services and the full benefits of innovation".
Regulators are also expected to levy fines in separate investigations into Google's Android mobile-phone software - possibly as soon as next month - and the AdSense advertising service.