But it was not her performance that had many people talking but rather what fans claimed was her fuller derriere, with many speculating the pop star had undergone bottom enhancement surgery or was possibly wearing padding on stage.
Hating that she was now the butt of jokes about her looks, with some bringing up Nicki Minaj comparisons, Madonna posted on Thursday, writing: "Desperately seeking no one's approval".
'Entitled to Free Agency Over My Body Like Everyone Else, ' she also wrote in the caption.
Although she did not directly mention the butt implants rumors, it does sound like this is what she was referring to.
Fans were delighted when superstar took to the Stonewall Inn stage on New Year's Eve and treated the crowd to acoustic versions of Like a Prayer and Elvis Presley'sCan't Help Falling in Love.
Furthermore, a video of her performance there became viral, causing many people to see it and give their opinion on it!
They started assuming her butt had changed after her New Year's Eve appearance. She later clapped back at the criticism.
The seven-time Grammy victor praised the LGBTQ community's efforts against "hatred, discrimination and, most of all, indifference", adding, "If we truly took the time to get to know one another we would find that we all bleed the same color and we all need to love and be loved".
2019 is shaping up to be a busy year for Madonna, who is reported to be releasing a new album and embarking on a world tour. "Let's remember who and what we are fighting for - ourselves, for each other".
The 60-year-old singer popped up at the Stonewall Inn, an iconic gay club in Greenwich Village, Manhattan, on Monday night. Think about that. Try it. Get outside your comfort zone.